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Recombinant DHX33 can rescue the defect in poly I:C-activated IFN production caused by DHX33 shRNA knockdown. (a) Immunoblot of endogenous DHX33 in D2SC mDCs (Control) and recombinant HA-DHX33 (sh+HA-X33) in D2SC mDCs in which endogenous DHX33 was selectively knocked down (shDHX33). (b) ELISA of IFN-β production by D2SC mDCs. IFN, interferon; mDC, myeloid dendritic cell; shRNA, small heteroduplex RNA.
