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. 2013 Mar 22;3(2):270–286. doi: 10.3390/biom3020270

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

The Q Exactive MS spectrum (a) and the HCD-MS/MS spectrum (b) of the glycopeptide QCN*QTSVCWCVNSVGVR (AA#118-134, N* denotes a conversion of N to D for the N-linked site Asn¹²⁰) from tumor-associated calcium signal transducer 2. The mass difference of 115 Da between y14 and y15 confirms the conversion Asn¹²° to Asp by PNGase F.