Figure 5.

Actin cytoskeletons are involved in Lm-induced MP generation by macrophages. (a) Actin cytoskeletons were involved in MP generation by macrophages. Peritoneal macrophages were incubated with CFSE-labeled Lm for 2 h, and then treated with cytochalasin D or blebbistatin, respectively. Twenty-four hours later, MPs in the supernatants were isolated and analyzed by a flow cytometer. The left panel shown here was a representative from three independent experiments. (b) Macrophages (5×10⁶) were treated with Lm or Lm/cytochalasin D or Lm/blebbistatin or control PBS for 24 h, and the released MPs were isolated from the supernatants. DCs were incubated with MPs and cocultured with CFSE-labeled T cells derived from Lm-infected mouse spleen for 72 h. T-cell proliferation was measured by a flow cytometer. The left panel shown here was a representative from three independent experiments. CFSE, carboxyfluorescein succinimidyl ester; DC, dendritic cell; Lm, Listeria monocytogenes; MP, microparticle; PBS, phosphate-buffered saline.