Figure 4.

Functional stability of recipient macrophages induced by allogeneic donor CD4⁺Foxp3⁺ Tregs in NOD-scid mice. (a) Allogeneic donor CD4⁺Foxp3⁺ Tregs and CD4⁺Foxp3⁻ T cells were sorted from Foxp3-GFP knock-in mice and confirmed by FCM. (b) After the adoptive transfer of isolated T-cell subsets for 7–8 days, the immunogenicity of the recipient resident macrophages was assessed by MLR. The proliferation of B6 CD4⁺ T cells that were induced by the allogeneic macrophages isolated from the NOD-scid mice that received no cells, CD4⁺Foxp3⁺ T cells, CD4⁺Foxp3⁻ T cells or both CD4⁺Foxp3⁺ T cells and CD4⁺Foxp3⁻ T cells was determined by a BrdU proliferation analysis in vitro. The data are presented as the mean±s.d. (N=4–5). **P<0.01 compared to the indicated groups. FCM, flow cytometry; MLR, mixed lymphocyte reaction; Treg, regulatory T cell.