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BrDU proliferation assay. On mouse C2C12 myoblast proliferation, 24 h, 48 h and 72 h EPO treatment has no effect. Sample size was n = 6 for each group. Experiment was performed in duplicate. Data are represented as mean ± SEM. #Significantly different from 24 h, P < 0.001. **Significantly different from CTRL, P < 0.01.
