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. 2009 Mar 9;30(4):467–477. doi: 10.1038/aps.2009.16

Figure 7.

Figure 7

Dose-dependent manner of toxicity to normal cells. (A) The MTT assay was used to determine the viability of L-02 cells at various doses of ZD55-IL-24, ZD55-IL-24 +cisplatin, or cisplatin after 48 h of treatment. Data are presented as means ± SD of three independent experiments. (B) CPE assay. Normal L-02 cells were treated with ZD55-IL-24, ZD55-IL-24+cisplatin, or cisplatin at the indicated doses. 72 h later, the cells were stained with crystal violet. (C) Morphological evaluation. L-02 cells were treated with ZD55-IL-24, ZD55-IL-24+cisplatin, or cisplatin and cell death was analyzed by microscopy after 24 h and 48 h. (Original magnification: ×100) (D) Apoptotic cell staining. Normal L-02 cells were treated with ZD55-IL-24 plus cisplatin at the indicated dose. Two days later, the cells were incubated with Hoechst 33342 and the condensation and fragmentation of nuclei was observed under a fluorescence microscope (arrow) (Hoechst 33342 stain; original magnification: ×200).