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. 2009 Mar 9;30(4):467–477. doi: 10.1038/aps.2009.16

Figure 8.

Figure 8

Combinatorial treatment with cisplatin and ZD55-IL-24 did not significantly affect the survival of normal cells. (A) L-02 cells were treated with ZD55-EGFP (5 MOI), ZD55-IL-24 (5 MOI), cisplatin (3 μg/mL), ZD55-EGFP (5 MOI)+cisplatin (3 μg/mL), or ZD55-IL-24 (5 MOI)+cisplatin (3 μg/mL). Cell viability was determined by the MTT assay. Data are presented as means±SD of three independent experiments. (B) Apoptosis detection by annexin V binding assay. The L-02 cells were treated as in Figure 8A. 72 h later, the cells were harvested and stained with annexin V-FITC, which was immediately followed by flow cytometry. The percentage of apoptotic cells was calculated using CellQuest software. Each value represents the mean of 3 wells.