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HBx upregulated the expression of PCNA and Bcl-2. (A) Western blot analysis showed that the expression levels of PCNA and Bcl-2 were upregulated in L-O2-X cells. HBx was detectable in L-O2-X cells. (B) PCNA, Bcl-2 and HBx protein were downregulated by transfection with pSilencer 3.0-X plasmid encoding silencing RNA, which targets HBx mRNA in L-O2-X cells. (C, D) The histogram shows the results from applying Glyco Band-Scan software.
