Figure 4.

The upregulation of COX-2, mediated by HBx, contributed to proliferation and is shown by BrdU incorporation assay. Positive DAPI (4,6-diamidino-2-phenylindole dihydrochloride hydrate, Sigma) staining was shown by blue fluorescence in the nuclei of cells. Green fluorescence showed the number of BrdU positive cells. (A) The BrdU incorporation assay showed that proliferation was higher in the L-O2-X cells relative to L-O2 cells (^bP<0.05, L-O2-X vs L-O2 cells, Student's t test). (B) The histogram represents three independent experiments with standard errors, shown as error bars.