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. 2009 Apr 3;30(4):388–395. doi: 10.1038/aps.2009.25

Figure 4.

Figure 4

Reduction of G2A expression blocked LPC effects on Aβ1–42-induced neurotoxicity. (A) G2A mRNA expression in SH-SY5Y cells after the treatment with Aβ1–42 (10 μmol/L). (B) G2A mRNA expression in SH-SY5Y cells after transfected with siRNA specific for G2A (“G2A-siRNA”) or scrambled siRNA (“Scr-siRNA”) as the control. Expression of G2A was normalized to β-actin in each sample. Relative G2A expression was calculated as described under Materials and Methods. (C) The reduction of cell viability in SH-SY5Y cells exposed to LPC after transfection with siRNA. Following transfection with siRNA, the cells were incubated with different concentrations of LPC (0, 2, 10, and 50 μmol/L) for 48 h and cell viability was determined using a CCK-8 kit. (D) The cell viability in SH-SY5Y cells exposed to LPC and/or Aβ1–42 after transfection with siRNA. Following transfection with siRNA, the cells were incubated with different concentrations of LPC (0, 2, 10, and 50 μmol/L) and Aβ1–42 (10 μmol/L) for 48 h. Cell viability was determined using a CCK-8 kit. Data are expressed as means±SEM from three independent experiments.