Figure 3.
GLUT-4 and Akt expression in hypoxic cardiomyocytes. A: immunocytochemistry for GLUT-4 (400×); buff-colored appear on the cell plasma membrane and/or in the cytoplasm, suggesting that these cells were positive. B: fluorescent immunocytochemistry for Akt (200×); red fluorescent grains on the cell plasma membrane and/or in the cytoplasm suggested that they were positive. a: 500 μmol/L CoCl2-induced hypoxia without Gs-Rb1 incubation for 12 h; b: 200 μmol/L Gs-Rb1 incubation after hypoxia induced with 500 μmol/L CoCl2.