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. 2009 Mar 23;30(4):396–403. doi: 10.1038/aps.2009.2

Figure 4.

Figure 4

Expression of GLUT-4 contents in cells (C) and cell plasma membrane (PM) of cardiomyocytes using Western blot analysis. A shows a representative Western blot and B shows its polygram. Lane 1: 0 μmol/L Gs-Rb1, 2: 10 μmol/L Gs-Rb1, 3: 50 μmol/L Gs-Rb1, 4: 100 μmol/L Gs-Rb1, 5: 200 μmol/L Gs-Rb1, 6: 400 μmol/L Gs-Rb1, 7: 500 μmol/L Gs-Rb1, 8: 500 μmol/L ara A+200 μmol/L Gs-Rb1 and 9: 0.5 μmol/L wortmannin +200 μmol/L Gs-Rb1; all lanes contain 200 μmol/L CoCl2. β-actin served as an internal control. n=3 for all groups.