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. 2010 Aug 23;31(9):1223–1240. doi: 10.1038/aps.2010.147

Figure 1.

Figure 1

(A) Diminished transactivation of AP-1 luciferase reporter by combinations of SFN and EGCG. PC-3 AP-1 cells were seeded in six-well plates and treated with individual dietary factors, or with combinations of SFN and EGCG, as indicated (S25, sulforaphane 25 μmol/L; E20, EGCG 20 μmol/L; E100, EGCG 100 μmol/L). The AP-1 luciferase activity was measured relative to vehicle control (DMSO) after 24 h of incubation and normalized against protein concentration. Values represent mean± standard deviation for three replicates, and are representative of seven independent experiments. bP<0.05 vs vehicle control; eP<0.05, significantly different from each other. (B) Viability of the PC-3 AP-1 cells with the combinations of SFN and EGCG. PC-3 AP-1 cells were treated with individual dietary factors or with combinations of SFN and EGCG for 24 h or 48 h, as indicated, and treated with MTS assay reagent to ascertain cell viability (S25, sulforaphane 25 μmol/L; E20, EGCG 20 μmol/L; E100, EGCG 100 μmol/L). Values represent mean±standard deviation for six replicates, and are representative of three independent experiments. bP<0.05 vs control.