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. 2010 Aug 23;31(9):1223–1240. doi: 10.1038/aps.2010.147

Figure 2.

Figure 2

Temporal gene expression profiles elicited by combinations of SFN and EGCG. PC-3 AP-1 cells were treated for 6 h or 10 h with individual dietary factors, or combinations of SFN and EGCG, as indicated (S25, sulforaphane 25 μmol/L; E20, EGCG 20 μmol/L; E100, EGCG 100 μmol/L). RNA was extracted, transcribed into cDNA after ascertaining RNA integrity, and quantitative real-time PCR assays were performed for twelve genes at both time-points, as indicated, using beta-actin as the housekeeping gene. Values represent mean±standard deviation for three replicates of each gene, and are representative of two independent experiments.