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. 2012 Aug 27;9(5):390–398. doi: 10.1038/cmi.2012.28

Figure 3.

Figure 3

SAHA regulates Th17/Treg balance. (a) Cardiac transplant was performed with Foxp3−/− B6 mice as recipients (BALB/C→Foxp3−/− C57BL/6) as described in Figure 1a. Shown was the graft survival curve. (b) Naive CD4+ T cells were isolated from mice spleen, and polarized for Th17 differentiation in the presence of different concentration of SAHA. IL-17A expressing CD4+ T cells were measured by flow cytometry. Shown are representative of three separate experiments. (c) The effect of SAHA on the expressions of STAT3, RORγt and Foxp3 measured by western blots. Shown are representative of three separate experiments. (d) Gene expressions of IL-17A, IL-17F, STAT3, RORγt, Foxp3 and CTLA-4 in CD4+ T cells measured by qPCR. The data are expressed as mean±s.d. (n=3). *P<0.05, **P<0.01, compared with Th17 group. DMSO, dimethylsulfoxide; qPCR, quantitative PCR; SAHA, suberoylanilide hydroxamic acid; Treg, regulatory T.