Figure 6.

Flagellin stimulation was not inhibited by sST2. (a) iMDDCs were stimulated by flagellin (FliC, 100 ng/ml) for 24 or 48 h, and secreted IL-8 was determined by ELISA. The data are presented as the means of three experiments±s.d. (b) iMDDCs were stimulated by LPS (100 ng/ml), and the amount of IL-8 was determined as (a). Pre-treatment with sST2 decreased the amount of secreted IL-8 slightly. ELISA, enzyme-linked immunosorbent assay; IL, interleukin; iMDDCs, immature monocyte-derived dendritic cell; LPS, lipopolysaccharide; sST2, soluble ST2 protein.