Figure 1.

Multiplex RNase protection analysis of expression of nAChR subunit genes in parent HEK 293 cells and KXα7R1 cells. RNase protection assays were carried out as described in Methods. Total RNA from cells was hybridizedwith a combination of [³²P] labeled anti-sense probes corresponding to the nine rat nAChR subunit genes α2, α3, α4 (lane 1); α5, α6, β4 (lane 2); α7, β2, β3 (lane 3). The probe corresponding to human GAPDH gene was used as an internal and loading control (all lanes). The images of expression profiles of HEK 293 and KXα7R1 cell lines were from different experiments but all mRNA measurements were repeated at least 3 times with similar results.