Figure 3.

Production of IFN-γ in γδT cells in response to stimulation with rHA. (a) PBMCs were stained with antibodies specific for TCRγδ and IFN-γ after incubation with the recombinant QH-HA proteins (A/Bar-headed Goose/Qinghai/2005 H5N1). The TCRγδ-positive cells were gated, and the IFN-γ-secreting γδT cells were analyzed by flow cytometry. (b) The average percentages of IFN-γ⁺ γδ T cells from six independent experiments. *P<0.05. The horizontal lines represent the mean values. HA, hemagglutinin; IFN, interferon; PBMC, peripheral blood mononuclear cell; rHA, recombinant hemagglutinin.