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. 2013 Aug 5;10(6):463–470. doi: 10.1038/cmi.2013.26

Figure 5.

Figure 5

rHA-induced activation of γδ T cells is not TCRγδ- or NKG2D-dependent. (a) ELISA analysis of the binding of rHAs to TCRγ9δ2 (OT3)-Fc proteins. (b) Flow cytometry analysis of the binding of an anti-TCRγδ monoclonal antibody and an anti-NKG2D monoclonal antibody to γδ T cells. (c) Confocal microscope scanning analysis of the binding of an anti-TCRγδ monoclonal antibody and an anti-NKG2D monoclonal antibody to γδ T cells. FITC-labeled goat-anti-mouse IgG was used as the secondary antibody. Scale bar=100 µm. (d) Antibodies against TCRγδ or NKG2D did not block rHA binding to γδ T cells. HA, hemagglutinin; rHA, recombinant hemagglutinin.