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Sialic acid receptors mediate the binding of rHA to γδ T cells. (a) Flow cytometry analysis showed that γδ T cells lacked expression of TLR2, TLR3, TLR4 and NKp46. (b) Flow cytometry analysis of α-2,3 and α-2,6 SA expression before (upper panel) and after (lower panel) neuraminidase digestion. (c) Flow cytometry analysis of rHA binding on γδ T cells before (left) and after (right) neuraminidase digestion. NK, natural killer; rHA, recombinant hemagglutinin; TLR, Toll-like receptor.
