Table 2. Primers used for screening and subtyping of HLA-A*02 alleles.

Primera	Sequences (5′–3′)	Intron/exon	Nucleotide locationb	Ref.
For main-screening
78C-FW1c	CTCGCCCCCAGGCTCC	Intron 1, exon 2	(120–130), 74–78	12
78C-FW2c	CCTCGTCCCCAGGCTCC	Intron 1, exon 2	(119–130), 74–78
78T-FW	CCTCGTCCCCAGGCTCT	Intron 1, exon 2	(119–130), 74–78	19–22
A-I3-RV	GTCCCAATTGTCTCCCCTC	Intron 3	(83–65)	24d
77G-FW	TCCTCGTCCCCAGGCTG	Intron 1, exon 2	(118–130), 74–77
538A-RV	GTGCTTGGTGGTCTGAGCT	Exon 3	556–538
hB2M-FW	CCGATATTCCTCAGGTACTC			12d
hB2M-RV	ACACAACTTTCAGCAGCTTAC			12d
For sub-screening
318A-FW	GACGGGGAGACACGGAAA	Exon 2	301–318
555A-RV	GCCGCCTCCCACTTGT	Exon 3	570–555
149C-FW	CCCCGCTTCATCGCC	Exon 2	135–149
440G-RV	CGGAGGAAGCGCCC	Exon 3	453–440
412A-FW	GTTCTCACACCATCCAGATA	Exon 3	393–412
555G-RV	GCCGCCTCCCACTTGC	Exon 3	570–555
For sequencing or cloning
A-I1-FW	CCTCTGYGGGGAGAAGCAA	Intron 1	(28–46)	24d
A-I4-RV	CAGAGAGGCTCCTGCTTTC	Intron 4	(54–36)
Seq-I2-RV	TCGGACCCGGAGACTGTG	Intron 2	(81–64)
Seq-I2-FW	GTTTCATTTTCAGTTTAGGCCA	Intron 2	(148–169)
Seq-I3-FW	GGTGTCCTGTCCATTCTC	Intron 3	(507–524)
For ambiguity resolving
A-F5-FW	GATTCCCCAACTCCGCAG	5′-flanking region	(−198–(−181))
78T-RV	GAAGAAATACCTCATGGAGTGA	Exon 2	99–78
Seq-I1-RV	CTCATGGAGTGAGAGCCTGG	Exon 2, intron 1	89–74, (130–127)

^aGroup- or allele-specific primers are named (polymorphic position and nucleotide)-FW/RV, locus-specific primers are named (locus)-(intron/exon)-FW/RV and sequencing primers are referred to as Seq-(Intron/Exon)-FW/RV.

^bThe exon nucleotides are numbered according to the alignment of coding DNA sequences of HLA-A alleles in the IMGT/HLA database.² The 5′-flanking region and intron passages are shown in the brackets and numbered according to Kotsch et al.²⁵

^c78C-FW1 and 78C-FW2 were synthesized separately and mixed at a 1∶1 mole ratio before use and designated as 78C-FW.

^dWith 5′ nucleotide truncations from or additions to previously reported sequences.