Table 3. Reaction combinations for the screening and subtyping of HLA-A*02 alleles.

Reaction	Primer combinationa	Target allelesb	Cycling protocolc	Amplicon size (bp)
For main-screening			CP1(1 min)
78C/I3	78C-FW, A-I3-RV	GNA2 group		881 or 882
78T/I3	78T-FW, A-I3-RV	GA2 group		882
77G/538A	77G-FW, 538A-RV	A*02:236		688
Control	hB2M-FW, hB2M-RV	(Human B2M gene)		333
For sub-screening			CP2(30 s)
318A/555A	318A-FW, 555A-RV	SA2 group		510
149C/440G	149C-FW, 440G-RV	Partial SNA2 allelesd		516
412A/555G	412A-FW, 555G-RV	Another partial SNA2 allelese		178
For sequencing or cloning			CP1(2 min)
78C/I4	78C-FW, A-I4-RV	GNA2 group		1728 or 1729
78T/I4	78T-FW, A-I4-RV	GA2 group		1729
I1/I4	A-I1-FW, A-I4-RV	HLA-A		1820
For ambiguity resolving			CP1(30 s)
F5/78T	A-F5-FW, 78T-RV	Partial GA2 alleles		427

^aPrimer information is listed in Table 2.

^bGroup names are specified in Figure 1 and Table 4.

^cExpressed as Protocol(Elongation time); the cycling protocols are detailed in the section on ‘Materials and methods'.

^dAlleles A*01:01:13, A*03:01:12, A*11:01:34, A*24:02:58, A*25:01:07, A*26:20 and A*68:03:02.

^eAlleles A*01:01:13, A*03:01:12, A*03:89, A*11:01:34 and A*30:08.