Table 5. HLA-A*02 subtyping strategies based on group-specific amplification.

Case	Reaction patternsa		Groupingb	Subsequent treatmentsc
Main-screening1(gDNA)c
	78C/I3	78T/I3
Case 1	+	+	GNA2/GA2	(Below)
Case 2	+	−	GNA2/GNA2, GNA2/SA	(Below)
Case 3	−	+	GA2/GA2, GA2/SA	Cloning-sequencing(I1/I4, (Seq-I2-RV, Seq-I2-FW, Seq-I3-FW))
Case 4	−	−	SA/SA	(Below)
For case 1: Sub-screening1(78C/I3) and Sub-screening2(78T/I3)c
	318A/555A	149C/440G OR 412A/555G
Case 1.1	−	−	NA2/A2	PCR(78T/I4), Sequencing(78T/I4, (Seq-I2-RV, Seq-I2-FW, Seq-I3-FW))
Case 1.2	−	+	NA2/SNA2
Case 1.3	+	−	SA2/A2	Combining treatments for cases 1.1 and 1.4
Case 1.4	+	+	SA2/SNA2	PCR(78C/I4), Sequencing(78C/I4, (Seq-I2-RV, Seq-I2-FW))
For case 2: Main-screening2(gDNA) and Sub-screening1(78C/I3)c
	77G/538A	318A/555A
Case 2.1	−	−	NA2/NA2, NA2/ENA2
Case 2.2	−	+	NA2/SA2, SA2/SA2, SA2/ENA2	Cloning-sequencing(I1/I4, (Seq-I2-RV, Seq-I2-FW))
Case 2.3	+	−	A*02:236/NA2
Case 2.4	+	+	A*02:236/SA2	PCR(78C/I4), Sequencing(78C/I4, (Seq-I2-RV, Seq-I2-FW))
For case 4: Main-screening2(gDNA)c
	77G/538A
Case 4.1	−		ENA2/ENA2
Case 4.2	+		ENA2/A*02:236, A*02:236/A*02:236	PCR(I1/I4), Sequencing(I1/I4, Seq-I2-RV)

Abbreviation: gDNA, genomic DNA.

^aReactions are detailed in Table 3.

^bGroup names are specified in Figure 1 and Table 4.

^cExpressed as Function(Parameter), e.g., Sub-screening1(78C/I3) means Sub-screening1 operation with amplicons of reaction 78C/I3 as templates; PCR(I1/I4) means PCR operation according to reaction I1/I4; Sequencing(I1/I4, Seq-I2-RV) means sequencing of amplicons of reaction I1/I4 with primer Seq-I2-RV; and Cloning-sequencing(I1/I4, (Seq-I2-RV, Seq-I2-FW, Seq-I3-FW)) means cloning of amplicons of reaction I1/I4 and sequencing of colony PCR products of two separate clones and the original amplicons of reaction I1/I4 with primers Seq-I2-RV, Seq-I2-FW and Seq-I3-FW.