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. 2009 Mar 5;30(3):346–354. doi: 10.1038/aps.2009.3

Figure 5.

Figure 5

Pan-caspase inhibitor or caspase-8 inhibitor, but not caspase-9 inhibitor, is capable of preventing the GA3-induced apoptosis. After pretreatment with 50 μmol/L pan-caspase inhibitor Z-VAD-FMK, or caspase-8 inhibitor Z-IETD-FMK or caspase-9 inhibitor Z-LEHD-FMK for 2 h, respectively. HL-60 cells were exposed to 2 μmol/L GA3 for 4 h and apoptosis was assayed by DNA fragmentation (A) and flow cytometry after staining with PI (B). (C) GA3-treated HL-60 cells, with or without Z-IETD-FMK pretreatment, were collected and PARP, procaspase-3, -8 and -9 and Bid protein expression were observed by Western blotting.