Table 3. Temperature dependence of mechanical and morphogenetic parameters.
| Process | Parameter (X) | X(16°C)/X(26°C) (LB, UB)1 |
| Morphogenesis | tc (time for blastopore closure) | 2.73 ** (2.29, 3.26) |
| tp (time for dorsal to ventral progression of involution) | 3.29 ** (2.75,3.94) | |
| RCP (tc/tp) | 0.83 ** (0.72, 0.94) | |
| Viscoelasticity | Compliance J(1) | 0.98 (0.85,1.13) |
| Compliance J(300) | 1.02 (0.9,1.16) | |
| β | 1.04 (0.88,1.24) | |
| Stimulated contractions | Contraction Duration | 3.36 ** (2.23,5.06) |
| Contraction Magnitude | 1.75 * (1.29,2.38) | |
| Tension duration (h) | 2.85 ** (2.26,3.6) | |
| Peak Apical Tension (f) | 1.37 (0.98,1.94) | |
| Endogenous actin dynamics | Duration2 | 1.32 ** (1.10, 1.57) |
*P≤0.05;
**P≤0.01:
Significant difference between 16 and 26°C for log transformed parameters.
1
Estimated mean and confidence interval for log-transformed parameters were determined using ANOVA (mechanics and actin) and Tukey's honestly significant difference criterion, or T-tests (morphogenesis).
Values were then transformed back to a linear scale to provide estimates of the lower and upper bounds (LB, UB) on the ratio.
2
Comparing 17° and 27°C. Ratios for the durations of actin contractions were 0.9 between 16.9°C and 21.3°C, and 1.5 between 21.3°C and 26.7°C.
To obtain confidence bounds here, we used ANOVA with temperature treatment as a categorical variable, and explant as a random factor; clutch was excluded because it was non-significant.