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TCS increased the percentage of T cells and the cytokine production in tumor-bearing mice. T cells derived from (a) the LNs and (b) spleen of TCS-treated tumor-bearing mice were stained with fluorescent antibodies. Foxp3 and cytokine production of CD4+ and CD8+ T cells were also analyzed by detecting intercellular expression. *P<0.05. LN, lymph node; TCS, trichosanthin.
