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. 2011 May 16;8(4):359–367. doi: 10.1038/cmi.2011.12

Figure 5.

Figure 5

TCS upregulated TSLC1 expression on 3LL tumor cells and CRTAM expression on T cells. (a) 3LL tumor cells were transfected with TSLC1 siRNA pool or siRNA-NC pool. After 48 h, the medium was refreshed, and cells were treated with TCS (50 µg/ml) or PBS. After a further 24-h culture, a group of 3LL cells was harvested, and the total cell protein was extracted for an independent western blot analysis of TSLC1. (b) Another group of 3LL cells was inactivated with MMC and cocultured with T cells isolated from the spleens of tumor-bearing mice. After 12 h, we collected the cocultured T cells and extracted the total RNA for analysis of the expression of CRTAM by real-time RT-PCR. (c) T cells from tumor-bearing mice treated with TCS or PBS were collected and cocultured with or without MMC-treated 3LL tumor cells for 6 h. The mRNA level of CRTAM was also detected by real-time RT-PCR. *P<0.05. CTRAM, class I-restricted T cell-associated molecule; MMC, mitomycin C; PBS, phosphate-buffered saline; TCS, trichosanthin; TSLC1; tumor suppressor in lung cancer 1.