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. 2011 Mar 21;8(4):341–347. doi: 10.1038/cmi.2011.7

Figure 2.

Figure 2

IL-12p70 production after DC maturation with different TLR agonists. (a) iDCs were matured with different TLR agonists as described in Figure 1. IL-12p70 secretion was measured in culture supernatants by ELISA. Only (LPS+poly(I:C))-DCs showed significantly higher production of IL-12p70 than the other DCs. (b, c) The optimal concentration of LPS to induce the synergistic effects was determined. iDCs were matured with various concentrations of LPS (0.01, 0.1 or 1 µg/ml) alone and in various combinations with poly(I:C) at 20 µg/ml. IL-12p70 was measured in supernatants during maturation and after CD40L stimulation. Only a concentration of 1 µg/ml LPS in combination with poly(I:C) enhanced the production of IL-12p70 during maturation as much as CD40L stimulation. The data are shown as the mean±s.d. (pg/ml) of triplicate cultures from two representative data sets from four independent experiments (*P<0.05). CD40L, CD40 ligand; DC, dendritic cell; Fla, flagellin; iDC, immature DC; Imi, imidazoquinoline; LPS, lipopolysaccharide; TLR, Toll-like receptor.