Figure 6.

L-selectin may be involved in the increase in surface CXCR4 expression in mouse neutrophils. (a–c) The surface L-selectin expression levels of neutrophils isolated from blood (a), lung tissue (b) and BAL fluid (c) at 24 h during LPS-induced lung injury were assessed by flow cytometry. The filled images show the staining using an isotype-matched control antibody. (d–f) Sulfatide induced the increase in surface CXCR4 expression in neutrophils isolated from mouse blood. The surface CXCR4 expression levels were examined before incubation (d) and after incubation for 1 h either with (f) or without (e) sulfatide. (g, h) Sulfatide induced shedding of L-selectin on neutrophils. Surface expressions of L-selectin on neutrophils were examined before incubation (g) and after incubation for 1 h with sulfatide (h). Representative histograms from one of three experiments that showed similar results are presented. BAL, bronchoalveolar lavage; LPS, lipopolysaccharide.