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. 2009 Jan 5;30(2):228–234. doi: 10.1038/aps.2008.19

Figure 2.

Figure 2

The effect of isoflurane on HO activity and HO-1 protein expression. (A) and (B) Isoflurane treatment and isofurane pretreatment on HO activity. ISO, cells treated with 2% isoflurane for 1 h. ISO-pre, cells incubated with isoflurane for 1 h, followed by 24 h exposure to medium alone. HO activity was measured at the end of incubation. HO activity was inhibited by SnPP. (C) Cells were exposed to 2% isoflurane for 1 h, followed by 24 h incubation with or without 1 μg/mL of LPS. (D)The Western blot is representative of 3 independent experiments. (E) Densitometric quantification of the result of (D). n=6–9 for A, B and C. Mean±SD. bP<0.05 vs cells without isoflurane preconditioning or LPS stimulation. eP<0.05 vs cells with LPS stimulation.