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Protein expression of Hsp60, vimentin, and NUP54 in human PASMCs. Human PASMCs were incubated with ET-1 (10−8 mol/L) or ET-1 (10−5 mol/L)+iptakalim (10−5 mol/L) or left untreated for 24 h. Proteins (50 μg each) from whole-cell lysates were run on 2-DE gels (A) or separated by SDS-PAGE and immunoblotted with antibodies against Hsp60, vimentin, NUP54, or GAPDH (B). We confirmed that the Western blot and proteomic results were identical.
