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. 2013 Feb 4;34(3):424–431. doi: 10.1038/aps.2012.181

Table 1. Cell culture conditions.

Cells Number/well V/well (mL) Plate Stimulation Read-out Incubation time
SPC 5.0×106 1 24-well ConA 2.5 µg/mL MTT assay, Griess reaction 24 h
DLNC 2.5×106 1 24-well MBP 10 ng/mL AP assay, ELISA, RT-PCR 24 h
PC 2.0×106 1 24-well LPS 10 ng/mL CV assay, Griess reaction 24 h
PC 2.0×106 1 24-well LPS 10 ng/mL Cytofluorimetry 30 min DHR+1 h
PC 4.0×106 1.5 24-well LPS 10 ng/mL TBARS assay 24 h
MGC 1.5×105 1 24-well ConASn 20% Cytofluorimetry 30 min DHR+1 h
Astrocytes 1.5×105 1 24-well ConASn 20% Cytofluorimetry 30 min DHR+1 h
Astrocytes 0.7×106 1.5 6-well ConASn 20% TBARS assay 24 h
Astrocytes 1.5×105 1 24-well ConASn 20% RT-PCR, immunoblot 24 h
C6 cells 1.5×105 1 24-well ConASn 20% Cytofluorimetry 30 min DHR+1 h
C6 cells 1.5×105 1 24-well ConASn 20% Immunoblot 24 h
C6 cells 1.0×106 1.5 6-well ConASn 20% TBARS assay 24 h
SCC 0.5×106 0.2 96-well No MTT assay, ELISA 24 h