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Cellular viability assay and proteasome activity assay. (A) MTT assay of cell viability. The proliferation of C6 glioma cells was inhibited after 3 h incubation with MG-132; at 24 h, the maximal inhibitory effect was reached. (B) Proteasome activity assay. Proteasome activity in C6 glioma cells was significantly inhibited at 3 h by 18.5 μmol/L MG-132. (cP<0.01 vs control group).
