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. 2011 May 2;32(5):611–618. doi: 10.1038/aps.2011.18

Figure 2.

Figure 2

Depletion of IRS-2 inhibits growth and reverses the transformation of R-/v-src cells. (A) R-/v-src cells were transfected with a specific siRNA for IRS-2 and either DMSO (Veh) or control siRNA (Con) as described in the Materials and methods. IRS-2 expression in cell lysates was detected by immunoblotting at 48 h after transfection. Cells were washed, transferred to SFM, and counted at the indicated times. The level of endogenous IRS-2 and IRS-1 was detected by immunoblotting, and protein expression was quantified by Image J (software from NCBI) and normalized to the internal control Grb-2. Mean±SD. bP<0.05 compared to control oligo-treated cells by ANOVA. (B) Colony formation of R-/v-src cells in soft agar after IRS-2 siRNA transfection. Cells were counted after 4 weeks. Colonies >125 μm were scored as positive. Mean±SD.