Figure 4.

ATX is crucial for Hep3B and Huh7 cells proliferation. (A) Hep3B or Huh7 cells were treated with IL-4 (10 ng/mL), CT (10 ng/mL), IL-4 (10 ng/mL) plus CT (10 ng/mL), ATX siRNA, or ATX siRNA plus IL-4 (10 ng/mL). Cells were grown for 48 h, and cell survival was measured with MTT staining. (B) ATX was knocked down by treatment of siRNA against ATX. Expression of ATX was assessed by Western blot. (C) Quantification of ATX expression in Hep3B cells treated with siRNA against ATX. Data are expressed as mean±SD of four independent experiments. ^cP<0.01 compared with untreated cells (control) (paired Student's t test).