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Phosphorylated Akt in HUVECs detected by Western blotting. (A) 10 μg/mL AA for 0, 15, 30, 60, or 120 min; (B) 5, 10 and 20 μg/mL AA for 30 min; (C) effects of LY294002 and wortmannin on phospho-Akt expression induced by AA in HUVECs. Mean±SD. n=5. bP<0.05, cP<0.01 for the AA group vs the control group; fP<0.01 for the LY294002+AA group and wortmannin+AA group vs the AA group.
