Figure 2.

Clau F promoted neurite outgrowth in PC12 cells. Quantitative analysis of band density was performed using three independent Western blotting analyses. (A) Clau F induction of neurite outgrowth was not concentration-dependent, and 10 μmol/L to 100 μmol/L concentrations could initiate neuritogenesis significantly after 2 d of incubation. (B) The effect of Clau F on the promotion of neurite outgrowth was examined based on morphology changes and increased expression of GAP-43. (C) The efficiency of neurite outgrowth promoted by Clau F was similar to that of NGF within 6 d of incubation. (D) The increased expression level of GAP-43 during long-term induction was evidenced both by immunoblotting and RT-PCR. ^bP<0.05, ^cP<0.01 vs control. β-Actin protein was used as a loading control.