Figure 3.

Clau F restrained cell proliferation without causing cell death. After treatment with different agents, the cells were incubated with MTT solution for 4 h and exposed to an MTT-formazan dissolving solution (a mixture of methanol and DMSO). The optical density (OD) was determined using an absorbance microplate reader (Molecular Devices, Toronto, Canada) set at a wavelength of 570 nm. The cell viability was expressed as a percentage of the OD value of the control cultures. (A) After 7 d of incubation, the control group showed exponential proliferation, whereas Clau F showed only moderate multiplication. (B) Cytotoxicity test of Clau F (10 μmol/L) on neural cells from the cerebral cortex of newborn SD rats. No significant difference was observed between the control culture at 0 d (a), the control culture at 7 d (b) or the Clau F-treated culture at 7 d (c). Morphological changes were observed using an inverted microscope. Mean±SEM. ^bP<0.05, ^cP<0.01 vs control.