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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Autoimmun. 2014 Feb 7;50:87–98. doi: 10.1016/j.jaut.2014.01.002

Figure 4.

Figure 4

BW iNKT cells provide help for spontaneous IgG secretion of B cells from B6, Sle1b and NZB/W mice, and α-GalCer augments production of IgG and IgG autoantibodies. Splenocytes or sorted CD19+ B cells with or without sorted iNKT cells were cultured for 10 days, and supernatants were collected for determination of IgM, IgG and IgG anti-dsDNA antibody concentrations. (A) IgM, IgG and IgG anti-dsDNA antibody concentrations from 10-day culture supernatants of 6 month old B6, Sle1b and NZB/W whole splenocytes. (B, C) IgM and IgG production from 6 month old mice, with B cells cultured with or without iNKT or Tcon cells from same strain (B) or different strains (C), in the presence of α-GalCer or not. (D) IgG anti-dsDNA antibody production from cell cultures of (B) detected by proteomic microarray. *, P<0.05; **, P<0.01; ***, P<0.001 (two-tailed Student’s t-test). Results (mean ± s.e.m.) are calculated from triplicate cultures from one representative experiment of two or three experiments with similar results.