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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Autoimmun. 2014 Feb 7;50:87–98. doi: 10.1016/j.jaut.2014.01.002

Figure 7.

Figure 7

Phenotype of infiltrating BW kidney T cells and production of IL-17. (A, B) Representative flow cytometric analyses of 6 month old BW kidney mononuclear cells (KMC) (A) and spleen cells (B). (C) Mean percentages of B, total T, iNKT and Tcon cells among gated lymphocytes. (D) Mean percentages of different subsets of iNKT cells among iNKT cells. (E) Mean percentages of CD4+CD8−, CD4−CD8+ and DN cells among BW kidney Tcon (left) and iNKT cells (middle), and percentages of total T, Tcon and iNKT DN cells among BW live kidney lymphocytes (right). (F) IFNγ and IL-17 production by BW KMC cells (5X105/ml) upon stimulation with anti-CD3 and CD28 mAbs. 5X105/ml KMC cells prepared from one or two kidneys from 6 month old BW mice were cultured in 96 well plates for 3 days. (G) IFNγ and IL-17 production by BW KMC cells with stimulation by α-GalCer. KMC (1X105) cells obtained from two 6 month old BW mice were cultured for 4 days with or without DCs (5X103), in the presence of α-GalCer (100 ng/mL) or not. Culture supernatants were collected for IFNγ and IL-17 measurements by Lumenix. ***, P<0.001 (two-tailed Student’s t-test). Bar graphs show mean ± s.e.m. Data are derived from six mice from three independent experiments (A-E), or from three independent experiments (F), or from two independent experiments (G).