Figure 1.Vps26a vps26b double mutant displays severe inhibition of lateral root initiation and does not accumulate a truncated form of the Vacuolar Sorting Receptor 1 (VSR1). (A) Root phenotype of 10-d-old seedlings from wild-type (WT), vps29-4 single and vps26a-1 vps26b-1 double mutant plants. Arrows indicate lateral roots. (B) Total protein extracts from wild-type (WT), vps35a vps35c, vps26a-2^−/− vps26b-1^+/− and vps26a-2^−/− vps26b-1^−/− mutant seeds were analyzed by immunoblotting for the expression of VSR1 and its truncated form. Molecular mass markers are indicated on the left in kDa.