Figure 2. Activation of Rab5 homologs is not required for the recruitment of VPS35 to endosomal membranes in Arabidopsis. Immunoblot analysis on total, cytosolic and microsomal proteins from wild-type (WT) and vps9a-2 seedlings using anti-VPS35a, anti-H⁺-ATPase and anti-cytosolic fructose-1,6-bisphosphatase (FBPase) antibodies. Cell fractionation was performed as previously described⁶ and H⁺-ATPase and FBPase were used as markers of membrane and cytosolic fractions, respectively. Molecular mass markers are indicated on the left in kDa.