Skip to main content
. 2013 May 6;34(7):901–911. doi: 10.1038/aps.2013.24

Figure 5.

Figure 5

Effect of Cur on LPS-induced intracellular ROS production and p47phox expression in VSMCs. Cell were pretreated with Cur (5, 10, or 30 μmol/L) for 100 min, and then incubated with 1 μg/mL LPS for another 2 h. Some cells were treated with Cur (30 μmol/L) alone for 2 h. The intracellular ROS levels were determined by DCFH oxidation (A). Cells were treated with Cur in the presence or absence of LPS (1 μg/mL) for 9 h. The expression of p47phox was detected by Western blotting (B). VSMCs were pretreated with or without DPI (20 μmol/L) for 1 h before the addition of Cur (30 μg/mL) for 1 h, and subsequently treated with LPS (1 μg/mL) for 2 h. The conditioned media were collected to measure the concentration of MCP-1 (C), TNF-α (D), and NO (E). Each bar represents the means±SEM of three independent experiments. cP<0.01 vs control; eP<0.05, fP<0.01 vs LPS; hP<0.05, iP<0.01 vs LPS+anti-TLR4; lP<0.01 vs LPS+Cur.