Skip to main content
. Author manuscript; available in PMC: 2015 Apr 16.
Published in final edited form as: Oncogene. 2013 Oct 28;33(42):4985–4996. doi: 10.1038/onc.2013.444

Figure 1. Knockdown of Aurora-A sensitizes tamoxifen-resistant cells to tamoxifen.

Figure 1

(A) Western blot was performed with indicated antibodies in a panel cell lines. Note: elevated Aurora-A was detected in tamoxifen-resistant MCF7-TamR and BT474 cells. (B) BT474 cells were transfected with two siRNAs of Aurora-A and control siRNA targeting eGFP. After 72 hours of incubation, immunoblot was carried out with indicated antibodies. (C–E) Aurora-A siRNA- and control siRNA-treated BT474 cells were treated with and without tamoxifen (0–2.0 μM). Total cell viability (C), apoptosis (D) and cell growth (E) were assessed by MTT assays, Annexin V/FACS and focus formation, respectively. Experiments were repeated three times, each experiment was triplicates. Error bars represent mean plus standard deviation. The asterisks denote significance (*P < 0.05 and ** P < 0.01).