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. Author manuscript; available in PMC: 2015 Apr 23.
Published in final edited form as: Oncogene. 2013 Oct 28;33(43):5100–5108. doi: 10.1038/onc.2013.447

Figure 2. Prohibitin mediates BASP1-dependent transcriptional repression of WT1 target genes.

Figure 2

V-K562 cells and BASP1-K562 cells were transfected with control siRNA or each of two different siRNAs against prohibitin (prohibitin siRNA 1 and prohibitin siRNA 2). 48 hours later cDNA was prepared. qPCR was performed to detect GAPDH, AREG, Ets-1 and BAX mRNA. Error bars are obtained from SDM of three independent experiments; *p<0.05 obtained by Student's t-test. Below, whole-cell extracts prepared in parallel after the transfection were immunoblotted with anti-prohibitin or anti-tubulin antibodies.