(A, B) Normal HSC-T6 cell percentage labeled with JC-1 in the inner membrane of mitochondria was 96.2%, and decreased to 59.7% when treated with 50 μmol/L ZK14 for 18 h. The decreased cells labeled with JC-1 revealed the loss of MMP and subsequently with the apoptosis of HSC-T6 cells. (C, D) Caspase 9 and 3 activity was measured and represented by ratios of ODtreated/ODcontrol after exposure to ZK14 for 18 h. After treated with 25, 50, and 100 μmol/L of ZK14 for 12 h, caspase 9 activity increased to 1.26, 1.55, and 2.36, and caspase-3 activity increased to 1.64, 1.85 and 2.38. n=3. Mean±SD. bP<0.05, cP<0.01 vs control.