Figure 3.

Effect of atorvastatin on inflammatory factors gene expressions at transcriptional levels in adipose tissue of MSG-induced obese mice with insulin resistance. Mice (n=5) were treated with atorvastatin (80 mg·kg⁻¹·d⁻¹) for 30 days. Non-obese mice (n=3) and vehicle control-treated obese mice (n=5) were normal and MSG respectively. (A) The products of semi-quantitative PCR were run on 2.0% agarose gels and stained with ethidium bromide. The expression level of each inflammatory factors mRNA was normalized to that of GAPDH shown in panel A. Atorvastatin inhibited the expression of TNF-α and IL-6 in the adipose tissue (B, C), meantime atorvastatin decreased the expression of IKK-β and NF-κB p65 (D, F). Futhermore, atorvastatin increased the IκB-α expression in adipose tissue (E). Values show means±SEM as ratio. ^bP<0.05 vs normal non-obese mice; ^eP<0.05, ^fP<0.01 vs control-treated obese mice.