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. 2010 Jan 5;31(1):51–65. doi: 10.1038/aps.2009.187

Figure 2.

Figure 2

Implications of using dexamethasone (DEX) as a media supplement (A, B). Impact of dexamethasone (DEX) (0–100 μmol/L) on mRNA expression of CYP3A9, ABCB1a, ABCB1b, and pregnane X receptor (PXR) in H411E and Iec-6 (C–H). Impact of dexamethasone (DEX) (500 nmol/L) on inducibility of CYP3A9 (C, D), ABCB1a (E, F), ABCB1b (G, H) and pregnane X receptor (PXR) (I, J) by pregnenolone 16α-carbonitrile (PCN) in H411E and Iec-6 cells. Data are mean ±SD of four experiments conducted in duplicate. For clarity, not all statistical analyses are given: bP<0.05, cP<0.01. For each transcript, a significant increase in expression was observed at concentrations ≤1 μmol/L when cells were incubated with pregnenolone 16α-carbonitrile (PCN) alone, which would not have been observed when dexamethasone (DEX) was incorporated into the media.