Figure 5.

Impact of typical activators on transporters. Effect of chenodeoxycholic acid (CDCA) (A–C), pregnenolone 16α-carbonitrile (PCN) (D–F), phenobarbital (PB) (G–I) and rifampicin (RIF) (J–I) in H411E, Iec-6 and primary hepatocytes. Data are mean±SD of four experiments conducted in duplicate. In cell lines, concentration dependency was investigated, whereas, in primary cells, time dependency was assessed at 1 μmol/L of each compound. Dotted lines indicate concentrations at which toxicity was observed following 5-day incubations with drug. For clarity, statistical analyses are given only for the lowest concentration at which a significant difference was observed: ^bP<0.05, ^cP<0.01.