Figure 2.

The effect of satraplatin on a clinically relevant concentration below its IC₅₀ value on p53-p21^waf1/cip1 protein expression at 48 h. Whole cell extracts were prepared from HCT116 (wt, p53^−/−, and p21^−/−), HCT15^mut, HT29^mut, and WiDr^mut cells treated with 5 and 10 μmol/L satraplatin for 48 h and subjected to Western blot analysis for the presence of p53 and p21^waf1/cip1 protein accumulation.